Modifications

Modifications

Biolegio supplies a long list of modifications, and this list is still growing. There are many ways to functionalize a primer, and they can be placed at different positions within the primer. Modifications can be added during DNA synthesis procedure: direct labelling, or indirect through the addition of an amino- or thiollinker.

Alexafluor dyes

Amino modifier

ATTO dyes

Biotin

Twice dyed probes

Cy labeling 

FAM 

Fluorescein 

FRET probes 

HEX

Inosin 

JOE  

 

LC replacement dyes

Licor dyes

Molecular beacons

NED / PET replacement dyes

Phosphorylation

Phosphorothioates

ROX

TAMRA

TET

Texas Red

Thiol modifier

Yakima Yellow